LIPID EXTRACT OF GRACILARIA SP. AS AN ANTI-APOPTOTIC CANDIDATE FOR CANCER THERAPY

It has been claimed that an Indonesian seaweed lipid extract possesses antibacterial properties. The mitochondrial pathway reported that a long fatty acid (more than 10 carbon atoms), which is known as the major component in seaweed lipid extract, acts as an apoptotic agent. It has also been claimed that unsaturated fatty acids can prevent apoptosis. on this study, we investigated the role of an Indonesian seaweed lipid extract on the mitochondrial pathway of apoptosis. Lipid isolation was the first step of the study, which was carried out using centrifugation and a chloroform-methanol-phosphate buffer 2:1:0,8. Rotary evaporators were used to separate the lipid extract from its solvent. Dry weight Gracilaria sp. yield extraction was 21,8%. By using several centrifugation techniques in SET buffer (0,25 M Sucrose, 0,5 mmol EDTA, and 10 mmol/L Tris base), pH 7,4, mitochondria were recovered from rat liver. By adding 10% (v/v) lipid extract to a suspension of mitochondria, toxicity testing from the lipid extract was undertaken. By using light microscopy, mitochondrial morphological damage was seen. Gracilaria sp. exhibits strong apoptotic activity

Novel Archaeal DNA Polymerase B from Domas Hot Spring West Java

Nine novel archaeal DNA polymerase genes from Domas Hot Spring, West Java have been cloned directly through the natural sample. The characterization of the genes showed that the genes are high homology to the DNA polymerase B of Crenarhaea phyla. Phylogenetic analysis of the amino acid sequences showed that the enzymes are grouped in a new branch from the other Crenarchaea’s DNA Polymerase B. 3D structure analysis of the enzymes show that the structures are closed to the structure of DNA Polymerase B1 from Sulfolobus solfataricus. The nine structures of the enzymes could be grouped into four different structures

Aktivitas Antibakteri Ekstrak Lipid Sargassum polycistum Terhadap Bacillus cereus dan Staphylococcus aureus

Rumput laut merupakan sumber daya hayati dari perairan Indonesia yang memiliki kandungan senyawa-senyawa bioaktif. Resistensi antimikroba merupakan permasalahan vital di dalam dunia medis. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak kasar lipid Sargassum polycistum di fasa metanol dan kloroform dalam menghambat pertumbuhan bakteri Bacillus cereus dan Staphylococcus aureus. Ekstraksi lipid menggunakan teknik sokhletasi dengan kombinasi pelarut metanol:kloroform (1:2/v/v). Pengujian antibakteri menggunakan metode difusi kertas cakram atau metode disc diffusion menurut Kirby-Bauer. Hasil ekstraksi lipid yang paling banyak diperoleh di fasa metanol sebanyak 11,2% (v/b) sedangkan fasa kloroformnya hanya sebanyak 3,8% (v/b). Fasa kloroform dari ekstrak kasar lipid Sargassum polycistum tidak memiliki daya hambat dalam pertumbuhan Bacillus cereus dan Staphylococcus aureus sedangkan fasa metanolnya memiliki aktivitas antibakteri dengan diameter zona bening sebesar 14 mm

The Effect of Different Matrix Bound on the Transesterification Activity of Immobilized PPD2 Lipase

Immobilization of thermostable lipase from Geobacillus thermoleovorans PPD2 (Lip-A) were carried out on Ni-NTA agarose and carboxymethyl chitosan. Free enzyme was obtained by heterologous expression in Escherichia coli as a host cell. The enzyme showed catalytic activity for transesterification reaction. Transesterification activity of immobilized lipase on Ni-NTA agarose was increased by three fold (75.04% conversion) compared to that the free enzyme (24.65%), while the activity of immobilized lipase on carboxymethyl chitosan was slightly decreased (19.86%)